Research Paper Volume 5, Issue 11 pp 813—824

Figure 4. Global loss of gene silencing of the HS-lacZ gene and the white minigene in the heterochromatin-located PEV reporter construct, BL2. (A) lacZ mRNA levels in 10 day and 50 day old BL2 flies after heat shock and mini-white mRNA levels (mWhite) in 10 day and 50 day old BL2 flies without heat shock. (B) lacZ mRNA levels in 10 day and 50 day old euchromatin control flies after heat shock and mini-white mRNA levels (mWhite) in 10 day and 50 day old BL2 flies without heat shock. mRNA levels were normalized to Gapdh1. qPCR data are from three independent biological replicates, representing three separate cohorts of 30 flies each (≥90 flies total) used for qRT-PCR. Error bars represent SD, *P < 0.05. There was age-related change in the level of mRNA expression for the HS-lacZ [40] or white minigene when the reporter construct is located in euchromatin.