Figure 1. lacZ expression in PEV flies. (A) Representative lacZ expression of Malpighian tubule cells from young, 10 day-old flies (upper panels) and old, 50 day-old flies (lower panels). Left panels show individual nuclei visualized with DAPI, shown in white; right panels show lacZ expression by X-gal staining of the same tubules, shown in blue. (B) Representative abdomens showing the blue lacZ expression of dorsal oenocytes in young, 10 day-old flies (left panel) and old, 50 day-old flies (right panel) from the BL2 line. The single white arrow in the left panel and the single white arrow in the right panel each point to an example of oenocytes positive for lacZ expression. Diffuse blue staining in central region of abdomen of 10 day-old fly in B is lacZ expression from bacteria in fly gut (