Research Paper Volume 5, Issue 10 pp 759—769
A DNA synthesis inhibitor is protective against proteotoxic stressors via modulation of fertility pathways in Caenorhabditis elegans
Figure 3. FUdR confers thermotolerance via germline signals that are independent of daf-12, daf-16, and hsf-1. A, FUdR-treatment significantly extends wild-type N2 survival at 35°C while it is slightly toxic to germline-deficient glp-1(e2141) mutants. Animals were reared at the restrictive temperature of 25°C and shifted to 35°C as young adults. B, FUdR-treatment significantly extends daf-12(rh61rh411) and daf-16(mu86) survival at 35°C to a similar extent as wild-type N2 animals. Animals were reared at 20°C and shifted to 35°C as young adults. C, FUdR-treatment significantly extends hsf-1(sy441) survival at 35°C to a similar extent as wild-type N2 animals. Animals were reared at 20°C and shifted to 35°C as young adults. In all experiments, FUdR was used at 100 μg/ml.