Figure 1.Flow Chart Describing Screening MethodologyFeeding RNAi clones corresponding to more than 400 transcription factors were retrieved from the Ahringer RNAi library. isp-1; Pgst-4::GFP double mutants (TJ564) and a Pgst-4::GFP control strain (CL2166), both containing GFP under the control of an oxidative stress-sensitive promoter, were screened in parallel for differential changes in reporter expression, development, adult size, fecundity and fertility. Fifteen transcription factors were identified that reproducibly and differentially altered some aspect of the isp-1 phenotype relative to the control strain (Table I). Seven of the identified RNAi clones significantly reduced isp-1 lifespan. Of these only two clones, targeting taf-4 and hif-1, significantly and differentially reduced the lifespan of isp-1, clk-1 and tpk-1 Mit mutants relative to control strains.
