Research Paper Volume 5, Issue 7 pp 539—550

Figure 5. Oxygen consumption in isolated soleus muscles is not affected by prior in vivo rapamycin treatment. After a one week recovery period, with continued rapamycin dosing, soleus muscle fibers were isolated form the animals represented in figure 4, permeabilized with digitonin, and subjected to respirometry in the presence of mitochondrial substrates and inhibitors using an Oxygraph-2K chamber (Oroboros). GM: Glutamate and Malate; ADP; Rot: Rotenone; Suc: Succinate; Ama: Antimycin A; TMPD/Asc: tetramethylphenylenediamine and ascorbate; Cyt C: Cytochrome c. Error bars show s.e.m; n=5.