Research Paper Volume 5, Issue 5 pp 347—356

Figure 1. Activation of FOXO1-AAA-ER induces expression of a miRNA cluster on chromosome X. (A) Illumina Bead-Array comparison of expression profiles of miRNA from HEK-293T cells expressing FOXO-AAA-ER with or without an 8- hour treatment with 4-hydoxitamoxifen (inducer). The position of the indicated miRNAs on the blot is marked. The expression of the miRNAs outside of the indicated boundaries has changed by a factor of 4 or more. (B) Comparison of expression profiles of miRNA from HEK-293T cells expressing FOXO-AAA-ER with or without treatment with 4-hydoxitamixifen using Illumina next-generation sequencing. The position of the indicated miRNAs on the blot is marked. (C) Schematic representation of genomic localization of the differentially expressed miRNA genes. A fragment of X chromosome is shown annotated in UCSC Genome Browser (genome.ucsc.edu) with genomic coordinates and the track of small RNAs.