Research Paper Volume 5, Issue 4 pp 234—269
Macromitophagy is a longevity assurance process that in chronologically aging yeast limited in calorie supply sustains functional mitochondria and maintains cellular lipid homeostasis
Figure 9. Under CR conditions, the atg32Δ mutation alters levels of several membrane lipid species in mitochondria, the ER and the PM. WT and atg32Δ strains were cultured in the nutrient-rich YP medium initially containing 0.2% glucose. Mitochondria, the ER and the PM were purified from WT and atg32Δ cells recovered on day 1, 2 or 4 of culturing. Following extraction of membrane lipids from purified mitochondria, ER and PM, various lipid species were identified and quantitated by mass spectrometry as described in Methods. Data are presented as means ± SEM (n = 3; *p < 0.01; ns, not significant).