Figure 9.Under CR conditions, the atg32Δ mutation alters levels of several membrane lipid species in mitochondria, the ER and the PMWT and atg32Δ strains were cultured in the nutrient-rich YP medium initially containing 0.2% glucose. Mitochondria, the ER and the PM were purified from WT and atg32Δ cells recovered on day 1, 2 or 4 of culturing. Following extraction of membrane lipids from purified mitochondria, ER and PM, various lipid species were identified and quantitated by mass spectrometry as described in Methods. Data are presented as means ± SEM (n = 3; *p < 0.01; ns, not significant).
