Figure 5.Gating scheme for ASCsHuman PBMCs upon staining with antibodies were first gated on lymphoid cells (not shown). (a) shows gating on single cells. (b) shows gating on live CD3 and CD14 negative cells. (c) shows gating on IgD negative cells. (d) shows gating on CD20 negative cells. (e) shows gating on cells expressing intermediate to high levels of CD19. (f) and (g) show gating on ASCs, which are CD28 high and CD37 high. F shows results of a young individual at baseline; (g) shows the sample from the same individual harvested 7 days after vaccination.
