Research Paper Volume 5, Issue 2 pp 120—129
How to measure RNA expression in rare senescent cells expressing any specific protein such as p16Ink4a
Figure 1. Expression of p16 at the single cell level measured by immunostaining with a p16 antibody followed by immunohistochemical detection. (A) Non-immortalized HDF (LF1) at early passage. (B) LF1 HDF passaged into senescence. (C) LF1 cells immortalized with telomerase (LF1/TERT) under conditions of exponential proliferation.