Research Paper Volume 5, Issue 2 pp 111—119
Temporal mTOR inhibition protects Fbxw7-deficient mice from radiation-induced tumor development
Figure 3. Inhibition of mTOR signaling sustains in tumors from rapamycin treated mice. (A) Detection of mTOR upstream and downstream signaling in the tumors from Fbxw7+/−p53+/− and p53+/− mice treated with rapamycin or placebo by Western blot assays with antibodies to p-AKT (Ser473), AKT, p-S6rp (Ser240 and Ser244), S6rp, Pten, and β-Actin. (B) Quantitative analysis of the total s6rp and p-s6rp levels in the blots showed in (A). Mean values (± standard deviation) were presented. **indicates p<0.001. (C) Quantitative analysis of the Pten levels in the blots showed in (A). Mean values (± standard deviation) were presented.