Research Paper Volume 5, Issue 2 pp 111—119
Temporal mTOR inhibition protects Fbxw7-deficient mice from radiation-induced tumor development
Figure 1. Radiation inhibits mTOR and its signaling in a FBXW7-depentend manner. mTOR and its signaling was assessed by Western blot assays with antibodies to p-mTOR (Ser2448), mTOR, p-S6rp (Ser240 and Ser244), S6rp, and β-Actin. (A) Detection of mTOR and its downstream signaling in HCT116 wild type and FBXW7−/− cells at different time points after single dose of 4Gy X-ray radiation. (B) Detection of mTOR and its downstream signaling in thymuses from wild type and FBXW7+/− mice that were collected at different time points after single dose of 4Gy X-ray radiation.