Research Paper Volume 5, Issue 2 pp 100—110

Figure 1. Survival plots for male and female Rb1^+/− (A) and Rb1^+/+ (B) mice, comparing control-fed mice to those fed eRapa in the diet starting at approximately 9 weeks of age (indicated by arrow). Control (black line) and eRapa (red line) survival curves are shown. The horizontal axes represent life span in days and the vertical axes represent survivorship. Rb1^+/− mice obtained from the NCI Mouse Repository were bred by the Nathan Shock animal core to obtain the cohorts of male and female mice used in this study. Genotype was confirmed as previously described [20]. eRapa mice were fed microencapsulated rapamycin-containing food (14mg/kg food designed to deliver approximately 2.24mg of rapamycin per kg body weight/day that achieved about 4 ng/ml blood [14]. Diets were prepared by TestDiet, Inc., Richmond, IN using Purina 5LG6 as the base [14]. Control diet was the same but with empty capsules. P values in (B) were calculated by the log-rank test.