Research Paper Volume 4, Issue 12 pp 899—916

Figure 5. Levels of pS6 and p-AKT in the livers of 10 months old mice: control, fasted, rapamycin. (A) Immunoblot analysis of protein lysates from livers of older (10 months old) females and males. Numbers indicate individual mice in each group. SL – standard loading,1 ug lysates from cultured cells was loaded onto each gel (left and right) and blots were over-exposed to obtain comparable intensities in standard loading lanes. (B) Quantified intensities of phosphorylated S6 (pS6) signal in livers of females and males. Left panel – female and male groups were comprised of all the animals from 3 groups (fasted, control and rapamycin) – n = 15 in female group and n = 18 in males group. Data are mean ± SE. (C) Quantified intensities of phosphorylated AKT (Ser 473) in livers of females and males. Data presented as described in legend B. (D) Correlation between levels of pS6 and p-AKT in livers from all the mice under study. (E) Comparison of older (~10 months old) female and male mice weights. Data present mean ± SE.