Figure 4.Casodex-induced TIF response in LNCaP cells is not due to inhibition of transcriptional activity or protein translationLNCaP cells were treated with cycloheximide (CHX, 20 μg/ml), actinomycin D (AMD, 0. 5 μg/ml), Casodex (100 μM), Casodex+CHX, or Casodex+AMD for 24 hr. (A), mRNA levels were assayed by RT-PCR; (B), protein levels were assayed by Western blot analysis; (C), cells were evaluated for a TIF response, based on the percentage of cells with >5 53BP1 foci/cell.
