Research Paper Volume 5, Issue 1 pp 18—36
Age-associated alterations in inducible gene transcription in human CD4+ T lymphocytes
Figure 3. Age-associated alterations in TCR-inducible gene expression in human peripheral blood CD4+ T cells. Total RNA prepared from anti-CD3 stimulated cells for the indicated times was converted to cRNA and hybridized to Illumina HumanRef-8 Expression BeadChip as described in Methods. The average expression level for each gene in the younger group (<65) was compared to the expression level for each gene in the older group (≥65). Genes whose average expression in Y and O groups differed by a Z-ratio >1.5, and p-value <0.05 were classified as being significantly different. The top 50 differentially up- (red) and down- (green) regulated genes after 2h (A) and 4h (B) of activation are shown. The total number of differentially up-regulated genes were 156 (2h) and 128 (4h). The total number of differentially down-regulated genes were 132 (2h) and 170 (4h).