Research Paper Volume 5, Issue 1 pp 67—83

Figure 6. AMO activities are reduced in Glc/MA cat cells relative to the WT control. (A) Measurements of AMO activities in crude extracts of WT and cat cells grown on Glc/MA. Activity is expressed as mU/mg protein. (B) Western blot analysis of AMO protein levels in cells grown for 16 h on Glc/MA. Cytosolic pyruvate decarboxylase Pyc1 was used as a loading control. (C) Quantification of AMO protein relative to Pyc1 of the blot shown in B. Data represent mean ± SD, n = 3. (D) CLS of WT and cat cells grown on Gly/MeOH/MA. Time point 0 h indicates time when cells were shifted to final medium. The number of colonies obtained after 16 h of growth was set to 100% viability. Experiments were repeated twice. Data represents mean ± SD, n = 3.