Research Paper Volume 5, Issue 1 pp 67—83

Figure 5. Cytochrome c peroxidase, superoxide dismutase and glutathione reductase activities in H. polymorpha WT and cat cells grown on different media. (A) Measurements of CCP activities in crude extracts of WT and cat grown on Glc/MA and Gly/MeOH/AS for 16 h. (B) Measurements of CCP activities in crude extracts of WT, cat, yap1 and cat yap1 cells grown on Gly/MeOH/AS for 4 h. (C) Superoxide dismutase isozyme profiling in WT cells. SOD activity was detected in crude extracts prepared from cells grown for 16 h on Glc/AS without pre-treatment (NT) or upon 30 min pre-incubation with 5 mM H₂O₂ or 5 mM KCN. (D) SOD activity in WT and cat cells grown for 16 h on Glc/MA or Gly/MeOH/AS. (E) SOD activity of in cat cells grown for 16 h in Gly/MeOH/AS detected without pre-treatment (NT) or after 30 min pre-incubation with 5 mM H₂O₂ or 5 mM KCN. (F) SOD activities in WT, cat, yap1, cat yap1 cells grown for 4 h on Gly/MeOH/AS. (G) Glutathione reductase activities in WT and cat cells grown in Glc/MA or Gly/MeOH/AS for 16 h. (H) CLS of ccp and cat ccp cells in Gly/MeOH/AS medium. Viability experiments were started 12 h after inoculation of the media. Experiments were repeated at least twice. Data represent mean activities ± SD, n = 3, ** - p<0.01. For native gels, representative gels are shown.