Research Paper Volume 4, Issue 11 pp 803—822

Figure 6. Electrical stimulation inducing apoptosis in cardiomyocytes derived from LMNA^R225X/WT &LMNA^{Frameshift/WT} iPSCs. Representative TUNEL assay and co-immunofluorescence staining of alpha-actinin in cardiomyocytes derived from (A) LMNA^WT/WT iPSCs. (B) LMNA^R225X/WT iPSCs. (C) LMNA^{Frameshift/WT} iPSCs. (D) LMNA^WT/WT iPSCs treated with mock shRNA. (E) LMNA^WT/WT iPSCs treated with shLMNA at baseline and after electrical stimulation. (F, G) Quantification of apoptotic cardiac differentiated iPSCs by APO-BrdU TUNEL assay. The percentage of cardiomyocytes with apoptosis was determined by FACS analysis by FL-1 positive gating. Unpaired t-test was performed between treatment and baseline n=3-5, *p-value<0.05.