Modeling of lamin A/C mutation premature cardiac aging using patient-specific induced pluripotent stem cells

Chung-Wah Siu; Yee-Ki Lee; Jenny Chung-Yee Ho; Wing-Hon Lai; Yau-Chi Chan; Kwong-Man Ng; Lai-Yung Wong; Ka-Wing Au; Yee-Man Lau; Jinqiu Zhang; Kenneth Weijian Lay; Alan Colman; Hung-Fat Tse

doi:doi:10.18632/aging.100503

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Research Paper Volume 4, Issue 11 pp 803—822

Modeling of lamin A/C mutation premature cardiac aging using patient-specific induced pluripotent stem cells

Figure 5. Electrical stimulation inducing nuclear abnormality in cardiomyocytes derived from LMNA^R225X/WT &LMNA^{Frameshift/WT} iPSCs. (A) Representative immunofluorescence staining of cardiomyocytes derived from LMNA^WT/WT iPSCs, LMNA^R225X/WT iPSCs, LMNA^{Frameshift/WT} iPSCs, LMNA^WT/WT iPSCs treated with mock shRNA, and LMNA^WT/WT iPSCs treated with shLMNA at baseline and after electrical stimulation. (B) The percentage of cardiomyocytes showed nuclear blebs.