Research Paper Volume 4, Issue 11 pp 742—754
Aging affects epidermal Langerhans cell development and function and alters their miRNA gene expression profile
Figure 2. Aging inhibited LC maturation. Epidermal cells were cultured in RPMI for 48h, and then stained with anti-Langerin, MHCII, CD80, CD86 antibodies, and analyzed by flow cytometry. The expression of MHCII and co-stimulatory molecules was determined by the ratio (A) and mean fluorescence intensity (MFI) (B). Data are shown as mean ± SEM of 6-10 mice each group.