Research Paper Volume 4, Issue 9 pp 648—659

Figure 5. STAT-4 phosphorylation is altered in CD4+ T cells from aged subjects. A. Histograms depict the phosphorylation of STAT-4 in naïve CD4+ T cells from aged and young at 0, 20, 60and 90 m after stimulation with IL-12. Graph is representative of 12 such experiments. B. Bar graph depicts the mean fluorescence intensity (MFI) of phosphorylation of STAT-4 in aged and young CD4+ T cells before and after activation with IL-12. Data is mean +/− S.E. of 12 different aged and young subjects. C. Bar graph also depicts phospho STAT-4 levels, the same as B using in cell ELISA. D. Western blot represents the level of non-phosphorylated STAT-4 in aged and young CD4+ T cells. Data represents pooled samples from 5 different aged and young subjects. E. Bar graph also depicts total STAT as in D using in cell ELISA. F. Graph depicts the level of IL-21 after treatment with JAK-2 inhibitor in aged CD4+ T cells. G. Graph depicts the level of IFN-γ after treatment with JAK-2 inhibitor in aged CD4+ T cells. Data is mean +/− S.E. of 6 different aged subjects.