Research Paper Volume 4, Issue 6 pp 436—447

Figure 1. AS101 increases SIRT1 levels in vitro and in vivo. (A-C) AS101 increases SIRT1 expression in several cell lines. For in vitro studies, AS101 and PBS were added to cultures of HEK293 (A), HL-60 (B) and Rin-5f (C) cells at a concentration of 0.1-2.5μg/ml, for 48 hours. Each experiment was done in three independent replicates and representative blots are shown. (D-E) AS101 increases SIRT1 expression in healthy rat livers. For the in vivo assay, healthy rats were injected daily i.p with AS101 or PBS at different concentrations for 14 days (0.25-1mg/kg AS101(D)) and for different periods of time (0.5 mg/kg) (E) (n=4 for each group.) SIRT1 levels in protein extracts from cell lines or rat liver, were measured by western blot analysis with anti- SIRT1 antibodies; actin was used as a loading control.