Research Paper Volume 4, Issue 6 pp 417—429
DNA binding residues in the RQC domain of Werner protein are critical for its catalytic activities
Figure 7. WRN RQC mutants are not able to stimulate NEIL1 incision activity. (A) NEIL1 incision activity in the presence of WRN mutants. 1 nM of NEIL1 was incubated with 0, 1, 2 or 4 nM of WRN wild type (lane 3 to 5) or WRN variants (lane 6 to 8, R993A; lane 9 to 11, F1037A; lane 12 to 14, R993A/F1037A) in the presence of 0.5 nM of DNA substrate at 37 °C for 10 min. Reaction products were separated on 10 % denaturing polyacrylamide gel. (B) Quantitative analysis of (A). Experiments were repeated at least three times, error bars represent ± SD.