Research Paper Volume 4, Issue 5 pp 368—374

Figure 5. Integrin α5β1 and EGFR share common signal pathways in regulating A431 cell proliferation. (A) Western-blot analysis of cell lysates proteins was perfomed as described in Fig. 4. The blots were probed with 1:1000 dilution of the listed antibodies. (B) Inhibition of Akt and Erk1/2 lead to a strongly reduced proliferation of A431 cells. Cells were incubated in serum-reduced medium, containing 25 μM of PI-3K inhibitor LY294002 (Sigma) or Erk inhibitor PD98059 (Calbiochem) or 0,5% DMSO, for 1 h and seeded in complete medium for 48 h whereupon the cells were harvested and quantified. (C) Inhibition of Akt (but not Erk1/2) induced significant apoptosis in A431 cells. Cells were treated as in indicated in (B), then fixed and prepared for flow cytometry as indicated in “Materials and methods”. FACS analysis was performed on a FACS-Calibur (Bekton Dickinson). Shown are typical experiments.