Research Paper Volume 4, Issue 5 pp 368—374
Integrin α5β1 simultaneously controls EGFR-dependent proliferation and Akt-dependent pro-survival signaling in epidermoid carcinoma cells
Figure 2. Integrin α5 knock-down or inhibition of EGFR suppress proliferation of A431 cells. (A and B) Cells tranduced with control or α5 shRNA vectors were cultivated in serum-reduced medium for 24 h and seeded as described in “Materials and methods”. (C) Cells were cultivated in-serum reduced medium for 24 h, treated with 5 μM of the EGFR inhibitor, PD168393, at 37°C for 1 h, seeded in complete medium for 24-72 h and quantified as described in “Materials and method”. Control (untreated) samples contaned 0,5% DMSO. Shown are the mean ± SD of two independent experiments.