Priority Research Paper Volume 4, Issue 4 pp 256—269

Figure 7. Long-lived daf-2 mutants have decreased uterine masses with age. a, Aligned cross sections of a 20-day-old wild-type worm stained with pararosaniline and methylene blue. The aligned cross sections were resliced longitudinally in software (top) from the mid-pharynx (top left) to the tail (top right). Cross sections from the same worm containing the gonad are shown spaced 50 μm apart (i-viii) and are represented by blue bars in the longitudinal section (top). In some cross sections, the uterus has enlarged to take up nearly the whole diameter of the worm (iii, iv, vii, viii). Intestine (int), distal gonad arm (dga), proximal gonad arm (pga), and uterus (ut) are indicated. b, Maximum projection of 24 and 45-day-old long-lived daf-2 worms raised at 20 degrees and stained with DAPI. Large uterine masses, similar to those seen in old wild-type worms, are visible at 24-days-old, but are reduced in size at 45-days-old. Scale bar represents 100 microns.