Figure 2. Age-related growths require a germline, but not competent sperm. a, Maximum projection of 8 and 18-day-old wild-type worm raised at 25 degrees and stained with DAPI. Growths visible at both ages, though generally never as severe as old wild-type worms raised at 20 degrees. b, Maximum projection of 8 and 16-day-old fem-2 (no sperm) worms raised at 25 degree restrictive temperature and stained with DAPI. Masses are visible at both ages, similar to wild-type worms. c, Maximum projection of 18-day-old glp-4 (underdeveloped gonad and germline) worm raised at 25 degree restrictive temperature and stained with DAPI. No masses are visible. Scale bar represents 100 microns.