Research Paper Volume 4, Issue 3 pp 206—223

dSir2 deficiency in the fatbody, but not muscles, affects systemic insulin signaling, fat mobilization and starvation survival in flies


Figure 5. Fatbody dSir2 regulates dilp5 meditated Insulin Signaling. (A-C) Relative dilp5 levels in the heads of (A) whole body dSir2RNAi (B) fatbody dSir2RNAi and (C) muscle dSir2RNAi flies, with respective controls, under fed and starved conditions (n = 24). Relative expression of (D) dInR and (E) d4eBP in fatbody dSir2RNAi flies under fed and starved conditions (n = 24). (F) Starvation survival of fatbody dSir2 knockdown (pSw-S1106-gal4>+/+;dSir2RNAi + RU486) and control (pSw-S1106-gal4>+/+;dSir2RNAi - RU486) flies, fatbody dSir2 knockdown in chico heterozygote flies (pSw-S1106-gal4>ch+/-; dSir2RNAi + RU486) and (pSw-S1106-gal4>ch+/-; dSir2RNAi- RU486) (statistical significance indicated in supplementary figure 9) (n = 60).200 μM RU486 was used to knockdown dSir2 expression. Log Rank was used to plot survival curves and Mantel-Cox test was used for statistical analysis. Student's t-test and ANOVA were used to analyze statistical significance of the data (*, p < 0.05; **, p < 0.01; ***, p < 0.001 or mentioned otherwise).