Research Paper Volume 4, Issue 3 pp 206—223

dSir2 deficiency in the fatbody, but not muscles, affects systemic insulin signaling, fat mobilization and starvation survival in flies


Figure 3. dSir2 in the fatbody, but not in the muscles regulates fat metabolism. (A) RT-PCR to show knockdown of dSir2 in the fatbody (FB) of fatbody dSir2RNAiand body wall with muscles (BWM) of muscle dSir2RNAi, with respective controls (n = 24). (B-C) Total body triglyceride levels in (B) fatbody dSir2RNAi flies and (C) muscles dSir2RNAi flies (n = 36). (D) Triglyceride levels in the isolated fatbody of fatbody dSir2RNAi flies (n = 60). (E) Relative expression of fat metabolism genes lipase-3 (lip3), brummer (brmm), medium chain acyl CoA dehydrogenase (mcad), long chain acyl CoA dehydrogenase (lcad) and fatty acid synthase (fas) in fatbody isolated from fatbody dSir2RNAi flies (n = 24). 200 μM RU486 was used to knockdown dSir2 expression pSw-S1106-gal4>dSir2RNAi and pSw-MHC-gal4> dSir2RNAi flies. Student's t-test was used to analyze statistical significance of the data (*, p < 0.05; **, p < 0.01; ***, p < 0.001 or mentioned otherwise).