Research Paper Volume 4, Issue 3 pp 206—223

Figure 3. dSir2 in the fatbody, but not in the muscles regulates fat metabolism. (A) RT-PCR to show knockdown of dSir2 in the fatbody (FB) of fatbody dSir2^RNAiand body wall with muscles (BWM) of muscle dSir2^RNAi, with respective controls (n = 24). (B-C) Total body triglyceride levels in (B) fatbody dSir2^RNAi flies and (C) muscles dSir2^RNAi flies (n = 36). (D) Triglyceride levels in the isolated fatbody of fatbody dSir2^RNAi flies (n = 60). (E) Relative expression of fat metabolism genes lipase-3 (lip3), brummer (brmm), medium chain acyl CoA dehydrogenase (mcad), long chain acyl CoA dehydrogenase (lcad) and fatty acid synthase (fas) in fatbody isolated from fatbody dSir2^RNAi flies (n = 24). 200 μM RU486 was used to knockdown dSir2 expression pSw-S₁106-gal4>dSir2^RNAi and pSw-MHC-gal4> dSir2^RNAi flies. Student's t-test was used to analyze statistical significance of the data (*, p < 0.05; **, p < 0.01; ***, p < 0.001 or mentioned otherwise).