Research Paper Volume 4, Issue 3 pp 206—223

dSir2 deficiency in the fatbody, but not muscles, affects systemic insulin signaling, fat mobilization and starvation survival in flies


Figure 1. dSir2 activity increases in response to starvation and its absence decreases starvation resistance. (A) Starvation survival of dSir2 mutants (Sir22A.7.11) (p < 0.001) and (B) whole body dSir2RNAi (+ RU486) (p < 0.001), with respective controls (n = 60). (C) dSir2 transcript levels increase in response to 48-hours starvation in control (- RU486) but not in whole body dSir2RNAi(+ RU486) flies (n = 8/24). (D) Increase in dSir2 protein levels in control flies in response to 48-hours starvation. (E) Increase in NAD+ levels in response to 48-hours starvation in control flies (n = 36). 200 μM RU486 was used to knockdown dSir2 expression inpSw-tub-gal4>dSir2RNAi flies. Log Rank was used to plot survival curves and Mantel-Cox test was used for statistical analysis. Student's t-test and ANOVA were used to analyze statistical significance of the data (*, p < 0.05; **, p < 0.01; ***, p < 0.001 or mentioned otherwise).