Figure 1. dSir2 activity increases in response to starvation and its absence decreases starvation resistance. (A) Starvation survival of dSir2 mutants (Sir22A.7.11) (p < 0.001) and (B) whole body dSir2RNAi (+ RU486) (p < 0.001), with respective controls (n = 60). (C) dSir2 transcript levels increase in response to 48-hours starvation in control (- RU486) but not in whole body dSir2RNAi(+ RU486) flies (n = 8/24). (D) Increase in dSir2 protein levels in control flies in response to 48-hours starvation. (E) Increase in NAD+ levels in response to 48-hours starvation in control flies (n = 36). 200 μM RU486 was used to knockdown dSir2 expression inpSw-tub-gal4>dSir2RNAi flies. Log Rank was used to plot survival curves and Mantel-Cox test was used for statistical analysis. Student's t-test and ANOVA were used to analyze statistical significance of the data (*, p < 0.05; **, p < 0.01; ***, p < 0.001 or mentioned otherwise).