Figure 3.Inhibition of SPARC expression by RNA silen-cing in young and old rat SMPCs(A) The efficiency of silencing by siSPARC was evaluated by qPCR in SMPCs from young and old rats 2 days after siRNA transfection. Data are expressed as the mean ± SEM (n = 4, respectively). *p < 0.05 vs. control. (B) The efficiency of siSPARC silencing was evaluated by immunoblotting SMPC extracts prepared from young and old rats 2 days after siRNA transfection. (C) Quantitative analysis of immunoblotting data. Data are expressed as mean ± SEM (n = 3, respectively). *p < 0.05 vs. control.
