Figure 7.Nitrotyrosine western blot analysis of 2D gel electrophoresis of young and old plantaris/FHL lysate following 5 days of reperfusionPlantaris/FHL lysates (180 μg) from (A) young (6-7 months) or (B) old (24-27 months) I/R tissues were separated by 2D gel electrophoresis. Nitrotyrosine immunoblotting was used to select nitrotyrosine modified proteins from a duplicate gel for mass spectrometry and identification.
