Research Paper Volume 3, Issue 8 pp 754—767

Figure 7. Measurement of ETC complex activities from young, middle aged and old WT and dwarf mouse kidney mitochondria. Complex enzyme activities were measured spectrophotometrically as described in Methods. All activity results are the average of 4 assays from the pooled samples ± SEM for each age group. Citrate synthase activities were used to normalize mitochondrial proteins. Activities for young (4-5 months), middle-aged (10-12 months), and old (20-26 months) WT and dwarf kidney ETC CI-CV are plotted as follows: (A) CI activity. Coefficients of variance for WT and dwarf were 3.9 % and 10.8% (young), 5.6% and 9.5% (middle-age), and 4.9% and 9.2% (old), respectively. (B) CII activity. Coefficients of variance for WT and dwarf were 2.6% and 7.5% (young), 6.5% and 3.6% (middle-age), and 6.8% and 7.7% (old), respectively. (C) CIII activity. Coefficients of variance for WT and dwarf were 13.7% and 12.9% (young), 10.2% and 8.8% (middle-age), and 12.7% and 4.3% (old), respectively. (D) CIV activity. Coefficients of variance for WT and dwarf were 9.9% and 4.8% (young), 6.8% and 8% (middle-age), and 4.6% and 7.7% (old), respectively. (E) CV activity. Coefficients of variance for WT and dwarf were 5.5% and 8% (young), 3.4% and 6.1% (middle-age), and 7.5% and 9% (old), respectively. * - p<0.05 compared to young, ** - p<0.001 compared to young, † - p<0.05 compared to middle-aged, ‡ - p<0.05 compared to WT, and ‡‡ - p<0.001 compared to WT.