Research Paper Volume 3, Issue 8 pp 754—767

Figure 1. Measurement of ETC complex enzyme activities from young, middle aged, and old WT and dwarf mouse heart mitochondria. Complex activities were measured spectrophotometrically as described in Methods. All activity results are the average of 4 assays from the pooled samples ± SEM for each age group. Citrate synthase activities were used to normalize mitochondrial proteins. Activities for young (4-5 months), middle-aged (10-12 months), and old (20-26 months) WT and dwarf heart CI-CV are plotted as follows: (A) CI activity. Coefficients of variance for WT and dwarf were 7.1% and 3.7% (young), 7.7% and 6.5% (middle-age), and 6.1% and 10.5% (old), respectively. (B) CII activity. Coefficients of variance for WT and dwarf were 8.3% and 6.3% (young), 7.5% and 3% (middle-age), and 6.9% and 10.5% (old), respectively. (C) CIII activity. Coefficients of variance for WT and dwarf were 6.1% and 13% (young), 3.8% and 9.5% (middle-age), and 6.2% and 3% (old), respectively. (D) CIV activity. Coefficients of variance for WT and dwarf were 1.8% and 4.6% (young), 4.3% and 3.3% (middle-age), and 2.4% and 8.7% (old), respectively. (E) CV activity. Coefficients of variance for WT were 6.1% and 10.7% (young), 3.2% and 6.6% (middle-age), and 9.3% and 8.3% (old), respectively. * - p<0.05 compared to young, ** - p<0.001 compared to young, † - p<0.05 compared to middle-aged, †† -p<0.001 compared to middle-aged, ‡ - p<0.05 compared to WT, and ‡‡ - p<0.001 compared to WT.