Research Perspective Volume 3, Issue 7 pp 692—697

Figure 1. RasGrf1 phosporylation in murine embryonic ES-D3 cells. Panel A. RasGrf1 protein phophorylation at Ser929 was detected by western blot after stimulation for 5 min with LIF (50 ng/mL), insulin (10 ng/mL), IGF-1 (100 ng/mL), and IGF-2 (100 ng/ml). Panel B. The total level of Ras-Grf1 protein. The experiment was repeated twice with similar results.