Research Paper Volume 3, Issue 7 pp 665—671
Immortalization of MEF is characterized by the deregulation of specific miRNAs with potential tumor suppressor activity
Figure 1. Characterization of MEF under 6T15 propagation regimen. (A) Proliferation curve of MEF expressed as cell doublings per passage. (B) Quantification of p19ARF, p16 and p21 transcripts per passage (each point represent one passage) normalized to that of MEF at passage 0. (C) p21 protein level at the various passages. The value reported under each lane represents the average of two independent experiments.