The Werner syndrome helicase protein is required for cell proliferation, immortalization, and tumorigenesis in Scaffold Attachment Factor B1 deficient mice

Sophie Lachapelle; Steffi Oesterreich; Michel Lebel

doi:doi:10.18632/aging.100300

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Research Paper Volume 3, Issue 3 pp 277—290

The Werner syndrome helicase protein is required for cell proliferation, immortalization, and tumorigenesis in Scaffold Attachment Factor B1 deficient mice

Figure 7. Induction of senescence by loss of Wrn helicase activity in Safb1-null MEFs. (A) Example of senescence-associated β-galactosidase staining in Safb1-null and Safb1-null/Wrn^Δhel/Δhel MEFs. Arrowheads point to positive cells. Magnification 100X. (B) Percentage of cells stained with senescence-associated β-galactosidase in wild type, Wrn^Δhel/Δhel, Safb1-null, and Safb1-null/Wrn^Δhel/Δhel MEFs. (Unpaired student t-test; *P < 0.045 and ** P < 0.022 compared to wild type MEFs). Bars represent SEM.