Research Paper Volume 3, Issue 3 pp 223—236
Gain of survival signaling by down-regulation of three key miRNAs in brain of calorie-restricted mice
Figure 2. Expression of three key MicroRNAs and Bcl-2 in CR mouse brain tissues. (A) In situ hybridization (ISH) detection of miRNAs (mmu-miR-34a, mmu-miR-30e and mmu-miR-181a-1*)in mouse brain tissues from both CR- and Ad lib-fed mice. Note increased hybridization signal of these miRNAs in the Ad lib group across all age groups, compared to CR. Graphical representations of densitometric analysis of miRNA (mmu-miR-34a, mmu-miR-30e and mmu-miR-181a-1*) hybridization in cortex and hippocampal regions. (B) Increased immunohistochemical staining of Bcl-2 in cortex and hippocampal region of CR, compared to Ad lib mice brain. Graphical representations of densitometric analysis of miRNA (mmu-miR-34a, mmu-miR-30e and mmu-miR-181a-1*) staining in cortex and hippocampal regions. (**p<0.01, **p<0.0001; all histograms represent average ± std.dev.)