Research Paper Volume 2, Issue 11 pp 815—822

Figure 1. Oct4 physically interacts with Npm1 and Tpt1 in ES cells. Immunofluorescence confocal microscopy in combination with in situ PLA, which detects protein-protein complexes, was used to explore interactions between Oct4 to Npm1 and Tpt1. Each detected complex is represented by a red dot. DNA was counterstained by Hoechst 33342 (blue). Scale bar represents 10 μm. (A) Complexes between endogenous Npm1 and Oct4 were found in the nucleoplasm of interphase cells. (B) Complexes between Npm1 and Oct4 during mitosis using an antibody specific to phosphorylated Npm1. (C) Complexes between endogenous Oct4 and Tpt1 in the nucleoplasm of interphase cells.