Research Paper Volume 2, Issue 10 pp 709—726

Figure 5. O₂^- inhibit growth arrest of stationary phase cells in G0/G1 in parallel with a shorter CLS. (A) CLS of wild type and sod2Δ cells in 2% glucose SC medium. (B) Levels of O₂^- in wild type and sod2Δ cells detected by DHE fluorescence at day 3 of medium depletion. C. Fraction of cells with visible buds in wild type and sod2Δ cells at indicated times of medium depletion. (D) CLS of wild type, sch9Δ and sch9Δ sod2Δ cells. (E) Levels of O₂^- detected by DHE fluorescence in wild type, sch9Δ and sch9Δ sod2Δ cells at day 3 of medium depletion. (F) Fraction of cells with visible buds in wild type, sch9Δ and sch9Δ sod2Δ cells at day 3 of medium depletion. (G) Dose-dependent effects of NAC on CLS in wild type and sch9Δ cells. (H and I) Dose-dependent effects of NAC on O₂^- detected by DHE (H) and fraction of cells with visible buds (I) in wild type and sch9Δ cells at day 3 of medium depletion. (J) Fraction of cells with visible buds in wild type cells and the catalase mutants cta1Δ and ctt1Δ at day 3 of medium depletion. (K) Fraction of cells with visible buds at day 3 of medium depletion that were treated or not treated with the anti-oxidant GSH beginning at day 0. (L) Effect of GSH in wild type cells on levels of O₂^- indicated by DHE fluorescence at day 3 of medium depletion.