Figure 4. Effects of SC medium containing 10% or 2% glucose on cell death in stationary phase. (A) % wild type cells cultured in 10% glucose SC medium stained with propidium iodide (PI) at day 2 of medium depletion. (B) DNA content of wild type cells cultured in 10% or 2% glucose SC medium at day 2 of stationary phase. Dotted line marks cells with a G1 content of DNA. (C-E) Electron micrographs of stationary phase cells cultured in 2% glucose SC medium undergoing apoptosis (C and D) or not undergoing apoptosis (E). (F) Number of particles in 10% glucose SC cultures at day 1 and 3 of medium depletion.