Research Paper Volume 2, Issue 10 pp 691—708
Regulatory roles of tankyrase 1 at telomeres and in DNA repair: suppression of T-SCE and stabilization of DNA-PKcs
Figure 7. Inhibition of tankyrase PARP activity decreases DNA-PKcs and increases tankyrase 1 protein levels. (A) Treatment of human lymphoblasts with the tankyrase PARP domain inhibitor XAV939 (0.5 or 1.0 μM) for 8 hr reduced DNA-PKcs protein levels to ~50% of the relative DMSO control. XAV939 treatment (1.0 μM) for 12 hr reduced DNA-PKcs levels to approximately 25% of the relative DMSO control. (B) Tankyrase 1 levels increased over DMSO controls in cells treated with XAV939, providing evidence that DNA-PKcs protein stability relies on the catalytic function of tankyrase 1. (RE) Relative Expression.