Research Paper Volume 2, Issue 10 pp 691—708

Figure 6. Proteasome inhibition facilitates DNA-PKcs protein recovery. Following siRNA depletion of tankyrase 1, WTK1 cells were treated (+) with the proteasome inhibitor MG132 for 2 hr at various times. Protein levels were measured at 10, 14 and 26 hr post transfection. When compared to untreated samples (-), protein levels of DNA-PKcs began to recover, suggesting tankyrase 1 prevents DNA-PKcs proteolytic degradation. Protein levels of tankyrase 1 were unaffected. Similar results were observed with the tankyrase PARP inhibitor XAV939 and MG132 treatment (Figure S4).