Research Paper Volume 2, Issue 10 pp 691—708

Figure 3. IR-induced chromosomal terminal deletions and telomere-DSB fusions are increased with tankyrase 1 siRNA knockdown. WTK1 lymphoblasts were treated on successive days with tankyrase 1 siRNA, or with the DNA-PKcs inhibitor (I), or with both, and irradiated (γ-rays or 1GeV ⁵⁶Fe) 48 hr after the second transfection. (A) Frequencies of terminal deletion, hallmarks of defective NHEJ, following IR exposure were elevated with either DNA-PKcs inhibition or depletion of tankyrase 1. (B) Frequencies of IR-induced telomere-DSB fusions, events characteristic of telomere uncapping, were elevated with inhibition of DNA-PKcs, and also with depletion of tankyrase 1. These data represent single experiments.