Figure 3.Aberrant protein O-GlcNAc cycling modestly enhances DAF-16 nuclear localization.DAF-16::GFP (translational fusion) nuclear localization in oga-1(RNAi) and ogt-1(RNAi) animals is modestly increased relative to control (vector) RNAi animals but much less than observed in age-1(RNAi) animals (upper panel). Lower panel is the quantification of DAF-16::GFP levels in intestinal cells upon RNAi inactivation of ogt-1, oga-1, age-1, and vector control.
