Research Paper Volume 2, Issue 10 pp 659—668

Figure 2. Doxorubicin (Dox) induces a dose-dependent hypertrophic response in rat H9c2 cardiomyocytes. Cells were treated with the indicated concentrations of doxorubicin for two hrs and cultured in fresh media for a further 24 hours prior to quantitation of cell volume (A) and total protein content per cell (B). Phase-contrast images of control untreated H9c2 myocytes (C) compared to cells treated with 1 μM doxorubicin (2 hr treatment followed by 24 hour incubation in fresh media, D). Bar = 5 μm; x 20 magnification.