Research Paper Volume 2, Issue 9 pp 582—596

ATM-independent, high-fidelity nonhomologous end joining predominates in human embryonic stem cells

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Figure 1. Description of the NHEJ-red repair cassette and processing of I-SceI-digested DNA. (A) Schematic of the NHEJ-red cassette. (B) Oct3/4 (green) positive hESCs display DsRed (red) 48 h after infection with a multiplicity of infection (MOI) of 30 with Ad-SceI adenovirus. DAPI shows nuclear staining.