Figure 3.Expression profiling of miRNAs that potentially target the SIRT1 3'-UTR during mESC differentiation. (A) 18 miRNAs from nine miRNA
families that potentially target the 3'-UTR of SIRT1 were induced during
mESC differentiation at the time SIRT1 protein was downregulated. Their
fold induction in d20 embryoid bodies above their expression in undifferentiated
mESCs was plotted on the y-axis, and the location of their seed binding
site in the 3'-UTR of mSIRT1 was plotted on the x-axis. (B-C), qRT-PCR of
miRNA expression relative to miR-16 from undifferentiated mESCs and
differentiating embryoid bodies of specific miRNAs that potentially target
SIRT1. Data are mean ± s.d. for four samples.
