Figure 4.TRIP6 and LPP co-immunoprecipitate with several shelterin components.(A) IP-Western blots on lysates made from
HTC75 cells obtained through retroviral transduction, stably expressing
MYC-TRIP6 (50kD) (the vector only control is shown on the left). The
lysates were used for immunoprecipitations with the antibodies listed on
top, and analyzed for the amounts of MYC-TRIP6 by Western blot with the
9E10 antibody. The Total fraction was ran alongside as indicated. The POT1
sera were the anti-epitope #4955 (left panel), and the anti¬baculovirus
POT1 #1048 (right panel). (B) Same as A, as with a MYC-LPP (66kD)
expressing HTC75 cells.
