Figure 3.POT1 interacts with TRIP6 in transient transfections.(A) IP-Western blots on lysates made from
transiently transfected 293T cells, to detect co-immunoprecipitation of the
transfected proteins. MYC-POT1 was co¬transfected with FLAG-TRIP6 or with
the FLAG vector, and the lysates were used for immunoprecipitations with
the 9E10 (anti-MYC) or M2 (anti-FLAG) antibodies, as shown on top. A Total
fraction (TOT) and beads only control (B) were run alongside. The
blot was probed with the 9E10 antibody, and the position of MYC-POT1 is
indicated by the arrow. The immunoprecipitated MYC¬POT1 by the FLAG
antibody is shown with the black triangle. (B) Same as A, except
that MYC-POT1∆OB is used in the co-transfection.
